Plant tissue culture is the process of hygienically multiplying plants by using artificial media,which can duplicate a large quantity of plants in a short period of time.All conditions-temperatures,light intensities,or humidity-significantly influence plant tissues to shoot new tips,roots,embryos,or calluses.

The main principle of plant tissue culture is that it needs to operate in a clean environment without microorganisms. Cleaning plant tissue is the first step in disinfecting. Then, putting plant tissues into new sterilized vessels and media. Moreover, they need to be stored in a suitable condition to influence plants to shoot their new part. Plant tissues need to gain nutrients and minerals from artificial media, which can be controlled by hormones that depend on specific purposes.